Exploring ribozyme conformational changes with X-ray crystallography
نویسندگان
چکیده
منابع مشابه
Accessing protein conformational ensembles using room-temperature X-ray crystallography.
Modern protein crystal structures are based nearly exclusively on X-ray data collected at cryogenic temperatures (generally 100 K). The cooling process is thought to introduce little bias in the functional interpretation of structural results, because cryogenic temperatures minimally perturb the overall protein backbone fold. In contrast, here we show that flash cooling biases previously hidden...
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ژورنال
عنوان ژورنال: Methods
سال: 2009
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2009.06.003